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recombinant mouse stem cell factor mscf  (PeproTech)


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    Structured Review

    PeproTech recombinant mouse stem cell factor mscf
    Recombinant Mouse Stem Cell Factor Mscf, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+stem+cell+factor+mscf/murine+stem+cell+factor/pm40080677-172-0-14
    Average 90 stars, based on 1 article reviews
    recombinant mouse stem cell factor mscf - by Bioz Stars, 2026-09
    90/100 stars

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    Recombinant:

    Article Title: A Connective Tissue Mast Cell-Specific Receptor Detects Bacterial Quorum Sensing Molecules and Mediates Antibacterial Immunity
    Article Snippet: .. Mast cells recovered in the pellet were resuspended in DMEM with 10% FBS, 100 U/mL penicillin, 50 μg/ml streptomycin and 25 ng/ml recombinant mouse stem cell factor (mSCF; Peprotech), and plated onto glass coverslips coated with 30 μg/ml fibronectin (Sigma). .. After 2 h of incubation at 37°C and 5% CO 2 , mast cells were loaded with 1:2000 Fluo-4, acetomethoxy ester (Molecular Probes) along with 0.02% Pluronic F-127 (1:1000; Molecular Probes) in calcium imaging buffer (CIB: NaCl 125 mM, KCl 3 mM, CaCl 2 2.5 mM, MgCl 2 0.6 mM, HEPES 10 mM, glucose 20 mM, NaHCO 3 1.2 mM, sucrose 20 mM, pH 7.4) for 30 min at 37°C, followed by wash ing once in CIB.

    Article Title: Mast-cell-specific receptor mediates alcohol-withdrawal-associated headache in male mice.
    Article Snippet: The pellets were resuspended in 2 mL MCDM, layered over 4 mL of an isotonic 70% Percoll solution (MilliporeSigma, St Louis, MO, USA), and centrifuged at 500 rcf for 20 min at 4 C. Purity of isolated mast cells was >95%, as determined from avidin staining. .. The mast cells were resuspended in DMEM with 10% FBS, 100 U/mL penicillin, 50 mg/mL streptomycin, and 25 ng/mL recombinant mouse stem cell factor (mSCF; Peprotech, Cranbury, NJ, USA) and plated onto glass coverslips coated with 30 mg/mL fibronectin (MilliporeSigma, St Louis, MO, USA). ..

    Article Title: Expansion of Quiescent Hematopoietic Stem Cells under Stress and Nonstress Conditions in Mice.
    Article Snippet: Hematopoietic stem cells (HSCs) are maintained in the quiescent state for protection from stress.. How quiescent HSCs expand in vivo under stress and nonstress conditions, however, is poorly understood.. Using the fluorescent ubiquitination-based cell cycle indicator (Fucci) mice, we analyzed quiescent and cycling HSCs in the bone marrow after transplantation and during development and aging.

    Article Title: Modular Layer-by-Layer Nanoparticle Platform for Hematopoietic Progenitor and Stem Cell Targeting.
    Article Snippet: StemSpan SFEM II serum-free, L-glutamine, recombinant human stem cell factor (SCF), recombinant mouse and human Fms-like tyrosine kinase 3/fetal liver kinase-2 (Flt3/Flk-2) ligands, recombinant mouse and human interleukin-3 (IL-3), recombinant mouse thrombopoietin (TPO), and human CD34+ cells were purchased from Stemcell Technologies (British Columbia, Canada). .. Recombinant mouse stem cell factor (mSCF) and recombinant human thrombopoietin (hTPO) were obtained from Peprotech (New Jersey). .. The 8-Chamber Lab-Tek II Chambered #1.5 German Coverglass system, wheat germ agglutinin Alexa Fluor 555 and 647, and Hoescht 33342 trihydrochloride trihydrate were purchased from Invitrogen (Massachusetts).

    Cell Culture:

    Article Title: Expansion of Quiescent Hematopoietic Stem Cells under Stress and Nonstress Conditions in Mice.
    Article Snippet: Hematopoietic stem cells (HSCs) are maintained in the quiescent state for protection from stress.. How quiescent HSCs expand in vivo under stress and nonstress conditions, however, is poorly understood.. Using the fluorescent ubiquitination-based cell cycle indicator (Fucci) mice, we analyzed quiescent and cycling HSCs in the bone marrow after transplantation and during development and aging.



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